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Molecular Devices

Enzyme Activation Analysis

Protocol File times-squared.spr     Download

This custom protocol fits the data for an activation profile of an enzyme.  In my case, plasminogen is activated to plasmin in the presence of the chromgenic substrate S2251 (or can use a fluorogenic substrate) and tissue plasminogen activator.  Since the zymogen (which has no chromogenic activity) is being activated to a protease (which will cleave the substrate S2251) the data produces curves in an upward direction. Since we need to know the increase of the rate from point-to-point, the data must be fitted to a "times-squared" model (similar to an acceleration model). This linearizes the data for analysis.  The slopes of the "times squared" fit are proportional to the rates of activation.

Showcases Data Analysis
Read Mode Any
SoftMax Pro Version 6.1
Primary Instrument Used SpectraMax M5e
Suitability Academic

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Added By Alan Stafford Added On 2011-12-19 20:09:03 UTC
Last Updated By Alan Stafford Last Updated On 2011-12-19 20:09:03 UTC
Downloads 82 Views 8,701


Thanks for uploading the protocol Alan. To help make the subject format a little more consistent with the other protocols already uploaded I removed the 'Protocol Contest() wrapper. i.e. changed the subject from 'Protocol Contest(Enzyme Activation Analysis)' to 'Enzyme Activation Analysis'. I hope you don't mind me doing so. Keith Thomas
2011-12-20 03:25:07 UTC

Congratulations you are our SoftMax Pro Contest winner for this quarter!!!
I will be sending you a separate email with more information shortly.
Kasia Proctor
2012-04-23 18:40:10 UTC

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