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Molecular Devices

Spectradrop Abs DNA Quant (iD3-iD5)

Protocol File Spectradrop Abs DNA Quant (iD3-iD5).spr     Download
Significance

THIS PROTOCOL IS FOR USE WITH SOFTMAX PRO 7.0.3 AND HIGHER.

 

Settings in this protocol have been revised to enable use of the SpectraDrop Micro-Volume Microplate with the SpectraMax iD3 and iD5 Multi-Mode Microplate Readers.


Protocol Use - Quantitation
Use this protocol to quantitate DNA using Molecular Devices' SpectraDrop Micro-Volume Plate. For samples exceeding 1000 ng/uL, dilution of the sample may be required for accurate quantitation. Samples whose concentrations are calculated to be greater than 1000 ng/uL will be flagged as out of range ('R') in the group tables.


This protocol is set up to take readings at 260 nm, 280 nm, and 230 nm.  The 260 nm reading is the only one required to quantitate DNA. The 280 nm and 230 nm readings are included for those who are accustomed to calculating 260:280 and 260:230 ratios for quality control purposes.
Showcases Data Analysis
Read Mode Absorbance
SoftMax Pro Version 7.0
Primary Instrument Used Other
Suitability Any

Molecular Devices Notes Section image is missing!

Added By Cathy Olsen Added On 2018-11-07 20:24:44 UTC
Last Updated By Cathy Olsen Last Updated On 2018-11-07 20:24:44 UTC
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